Western Blot:Article Title: OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis
Article Snippet: Antibodies list mouse monoclonal anti-LSD1 (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) CST Cat# 4218 rabbit polyclonal anti-LSD1 (Immunoblotting, ChIP-seq and ChIP-qPCR) BETHYL Cat# A300-215A rabbit polyclonal anti- OTUD7B (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) Proteintech Cat# 16605-1-AP mouse monoclonal anti- OTUD7B (immunoblotting) Proteintech Cat# 66276-1-Ig rabbit polyclonal anti-CoREST (Immunoblotting) CST Cat# 14567 mouse monoclonal anti- H3K9me2 (ChIP-seq and ChIP-qPCR) Abcam Cat#1220 rabbit polyclonal anti- H3K4me2 Millipore Cat# 07-030 rabbit polyclonal anti-HDAC1 (immunoblotting) Proteintech Cat# 10197-1-AP rabbit polyclonal anti-HDAC2 (immunoblotting) Proteintech Cat# 12922-3-AP rabbit polyclonal anti-USP22 (immunoblotting) Proteintech Cat# 55110-1-AP rabbit polyclonal anti-USP28 (immunoblotting) Proteintech Cat# 17707-1-AP rabbit polyclonal anti-Flag (immunoblotting) Proteintech Cat# 20543-1-AP rat monoclonal anti-HA (immunoblotting) Roche Cat# 3F10 mouse monoclonal anti-Actin (immunoblotting) Sigma Cat# A1978 mouse monoclonal anti-Myc (immunoblotting) CST Cat# 2276 rabbit polyclonal anti-Myc (immunoblotting) Proteintech Cat# 16286-1-AP mouse monoclonal anti-GFP (immunoblotting) Proteintech Cat# 66002-1-lg rabbit polyclonal anti-p62 (immunoblotting) Proteintech Cat# 18420-1-AP mouse monoclonal anti-Cyclin B (immunoblotting) CST Cat# 4135 mouse monoclonal anti-Cyclin E (immunoblotting) CST Cat# 4129 mouse monoclonal anti-Cyclin D1 (immunoblotting) Proteintech Cat# 60186-1-Ig rabbit polyclonal anti-CDK6 (immunoblotting) CST Cat# 14052-1-AP mouse monoclonal anti- Tubulin (immunoblotting) Proteintech Cat# 66240-1-lg rabbit polyclonal anti-PKD1 (immunoblotting) ABclonal Cat# A14200 rabbit polyclonal anti-RIN1 (immunoblotting) Proteintech Cat# 16388-1-AP rabbit polyclonal anti-RBBP8 (immunoblotting) Proteintech Cat# 12624-1-AP rabbit polyclonal anti-Keap1 (immunoblotting) Proteintech Cat# 10503-2-AP rabbit polyclonal anti-RASSF5 (immunoblotting) SAB Cat# 27727 rabbit monoclonal anti-Snail (immunoblotting) CST Cat# 3879 mouse monoclonal anti-His (immunoblotting) Proteintech Cat# 66005-1-Ig rabbit polyclonal anti-GST (immunoblotting) Proteintech Cat# 10000-0-AP mouse monoclonal anti-Lamin B1 (immunoblotting) Proteintech Cat# 66095-1-Ig mouse monoclonal anti- GAPDH (immunoblotting) Santa cruz Cat# sc32233 Table S6.
Immunofluorescence:Article Title: OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis
Article Snippet: Antibodies list mouse monoclonal anti-LSD1 (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) CST Cat# 4218 rabbit polyclonal anti-LSD1 (Immunoblotting, ChIP-seq and ChIP-qPCR) BETHYL Cat# A300-215A rabbit polyclonal anti- OTUD7B (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) Proteintech Cat# 16605-1-AP mouse monoclonal anti- OTUD7B (immunoblotting) Proteintech Cat# 66276-1-Ig rabbit polyclonal anti-CoREST (Immunoblotting) CST Cat# 14567 mouse monoclonal anti- H3K9me2 (ChIP-seq and ChIP-qPCR) Abcam Cat#1220 rabbit polyclonal anti- H3K4me2 Millipore Cat# 07-030 rabbit polyclonal anti-HDAC1 (immunoblotting) Proteintech Cat# 10197-1-AP rabbit polyclonal anti-HDAC2 (immunoblotting) Proteintech Cat# 12922-3-AP rabbit polyclonal anti-USP22 (immunoblotting) Proteintech Cat# 55110-1-AP rabbit polyclonal anti-USP28 (immunoblotting) Proteintech Cat# 17707-1-AP rabbit polyclonal anti-Flag (immunoblotting) Proteintech Cat# 20543-1-AP rat monoclonal anti-HA (immunoblotting) Roche Cat# 3F10 mouse monoclonal anti-Actin (immunoblotting) Sigma Cat# A1978 mouse monoclonal anti-Myc (immunoblotting) CST Cat# 2276 rabbit polyclonal anti-Myc (immunoblotting) Proteintech Cat# 16286-1-AP mouse monoclonal anti-GFP (immunoblotting) Proteintech Cat# 66002-1-lg rabbit polyclonal anti-p62 (immunoblotting) Proteintech Cat# 18420-1-AP mouse monoclonal anti-Cyclin B (immunoblotting) CST Cat# 4135 mouse monoclonal anti-Cyclin E (immunoblotting) CST Cat# 4129 mouse monoclonal anti-Cyclin D1 (immunoblotting) Proteintech Cat# 60186-1-Ig rabbit polyclonal anti-CDK6 (immunoblotting) CST Cat# 14052-1-AP mouse monoclonal anti- Tubulin (immunoblotting) Proteintech Cat# 66240-1-lg rabbit polyclonal anti-PKD1 (immunoblotting) ABclonal Cat# A14200 rabbit polyclonal anti-RIN1 (immunoblotting) Proteintech Cat# 16388-1-AP rabbit polyclonal anti-RBBP8 (immunoblotting) Proteintech Cat# 12624-1-AP rabbit polyclonal anti-Keap1 (immunoblotting) Proteintech Cat# 10503-2-AP rabbit polyclonal anti-RASSF5 (immunoblotting) SAB Cat# 27727 rabbit monoclonal anti-Snail (immunoblotting) CST Cat# 3879 mouse monoclonal anti-His (immunoblotting) Proteintech Cat# 66005-1-Ig rabbit polyclonal anti-GST (immunoblotting) Proteintech Cat# 10000-0-AP mouse monoclonal anti-Lamin B1 (immunoblotting) Proteintech Cat# 66095-1-Ig mouse monoclonal anti- GAPDH (immunoblotting) Santa cruz Cat# sc32233 Table S6.
Immunohistochemistry:Article Title: OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis
Article Snippet: Antibodies list mouse monoclonal anti-LSD1 (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) CST Cat# 4218 rabbit polyclonal anti-LSD1 (Immunoblotting, ChIP-seq and ChIP-qPCR) BETHYL Cat# A300-215A rabbit polyclonal anti- OTUD7B (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) Proteintech Cat# 16605-1-AP mouse monoclonal anti- OTUD7B (immunoblotting) Proteintech Cat# 66276-1-Ig rabbit polyclonal anti-CoREST (Immunoblotting) CST Cat# 14567 mouse monoclonal anti- H3K9me2 (ChIP-seq and ChIP-qPCR) Abcam Cat#1220 rabbit polyclonal anti- H3K4me2 Millipore Cat# 07-030 rabbit polyclonal anti-HDAC1 (immunoblotting) Proteintech Cat# 10197-1-AP rabbit polyclonal anti-HDAC2 (immunoblotting) Proteintech Cat# 12922-3-AP rabbit polyclonal anti-USP22 (immunoblotting) Proteintech Cat# 55110-1-AP rabbit polyclonal anti-USP28 (immunoblotting) Proteintech Cat# 17707-1-AP rabbit polyclonal anti-Flag (immunoblotting) Proteintech Cat# 20543-1-AP rat monoclonal anti-HA (immunoblotting) Roche Cat# 3F10 mouse monoclonal anti-Actin (immunoblotting) Sigma Cat# A1978 mouse monoclonal anti-Myc (immunoblotting) CST Cat# 2276 rabbit polyclonal anti-Myc (immunoblotting) Proteintech Cat# 16286-1-AP mouse monoclonal anti-GFP (immunoblotting) Proteintech Cat# 66002-1-lg rabbit polyclonal anti-p62 (immunoblotting) Proteintech Cat# 18420-1-AP mouse monoclonal anti-Cyclin B (immunoblotting) CST Cat# 4135 mouse monoclonal anti-Cyclin E (immunoblotting) CST Cat# 4129 mouse monoclonal anti-Cyclin D1 (immunoblotting) Proteintech Cat# 60186-1-Ig rabbit polyclonal anti-CDK6 (immunoblotting) CST Cat# 14052-1-AP mouse monoclonal anti- Tubulin (immunoblotting) Proteintech Cat# 66240-1-lg rabbit polyclonal anti-PKD1 (immunoblotting) ABclonal Cat# A14200 rabbit polyclonal anti-RIN1 (immunoblotting) Proteintech Cat# 16388-1-AP rabbit polyclonal anti-RBBP8 (immunoblotting) Proteintech Cat# 12624-1-AP rabbit polyclonal anti-Keap1 (immunoblotting) Proteintech Cat# 10503-2-AP rabbit polyclonal anti-RASSF5 (immunoblotting) SAB Cat# 27727 rabbit monoclonal anti-Snail (immunoblotting) CST Cat# 3879 mouse monoclonal anti-His (immunoblotting) Proteintech Cat# 66005-1-Ig rabbit polyclonal anti-GST (immunoblotting) Proteintech Cat# 10000-0-AP mouse monoclonal anti-Lamin B1 (immunoblotting) Proteintech Cat# 66095-1-Ig mouse monoclonal anti- GAPDH (immunoblotting) Santa cruz Cat# sc32233 Table S6.
Immunoprecipitation:Article Title: OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis
Article Snippet: Antibodies list mouse monoclonal anti-LSD1 (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) CST Cat# 4218 rabbit polyclonal anti-LSD1 (Immunoblotting, ChIP-seq and ChIP-qPCR) BETHYL Cat# A300-215A rabbit polyclonal anti- OTUD7B (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) Proteintech Cat# 16605-1-AP mouse monoclonal anti- OTUD7B (immunoblotting) Proteintech Cat# 66276-1-Ig rabbit polyclonal anti-CoREST (Immunoblotting) CST Cat# 14567 mouse monoclonal anti- H3K9me2 (ChIP-seq and ChIP-qPCR) Abcam Cat#1220 rabbit polyclonal anti- H3K4me2 Millipore Cat# 07-030 rabbit polyclonal anti-HDAC1 (immunoblotting) Proteintech Cat# 10197-1-AP rabbit polyclonal anti-HDAC2 (immunoblotting) Proteintech Cat# 12922-3-AP rabbit polyclonal anti-USP22 (immunoblotting) Proteintech Cat# 55110-1-AP rabbit polyclonal anti-USP28 (immunoblotting) Proteintech Cat# 17707-1-AP rabbit polyclonal anti-Flag (immunoblotting) Proteintech Cat# 20543-1-AP rat monoclonal anti-HA (immunoblotting) Roche Cat# 3F10 mouse monoclonal anti-Actin (immunoblotting) Sigma Cat# A1978 mouse monoclonal anti-Myc (immunoblotting) CST Cat# 2276 rabbit polyclonal anti-Myc (immunoblotting) Proteintech Cat# 16286-1-AP mouse monoclonal anti-GFP (immunoblotting) Proteintech Cat# 66002-1-lg rabbit polyclonal anti-p62 (immunoblotting) Proteintech Cat# 18420-1-AP mouse monoclonal anti-Cyclin B (immunoblotting) CST Cat# 4135 mouse monoclonal anti-Cyclin E (immunoblotting) CST Cat# 4129 mouse monoclonal anti-Cyclin D1 (immunoblotting) Proteintech Cat# 60186-1-Ig rabbit polyclonal anti-CDK6 (immunoblotting) CST Cat# 14052-1-AP mouse monoclonal anti- Tubulin (immunoblotting) Proteintech Cat# 66240-1-lg rabbit polyclonal anti-PKD1 (immunoblotting) ABclonal Cat# A14200 rabbit polyclonal anti-RIN1 (immunoblotting) Proteintech Cat# 16388-1-AP rabbit polyclonal anti-RBBP8 (immunoblotting) Proteintech Cat# 12624-1-AP rabbit polyclonal anti-Keap1 (immunoblotting) Proteintech Cat# 10503-2-AP rabbit polyclonal anti-RASSF5 (immunoblotting) SAB Cat# 27727 rabbit monoclonal anti-Snail (immunoblotting) CST Cat# 3879 mouse monoclonal anti-His (immunoblotting) Proteintech Cat# 66005-1-Ig rabbit polyclonal anti-GST (immunoblotting) Proteintech Cat# 10000-0-AP mouse monoclonal anti-Lamin B1 (immunoblotting) Proteintech Cat# 66095-1-Ig mouse monoclonal anti- GAPDH (immunoblotting) Santa cruz Cat# sc32233 Table S6.
Chromatin Immunoprecipitation:Article Title: OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis
Article Snippet: Antibodies list mouse monoclonal anti-LSD1 (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) CST Cat# 4218 rabbit polyclonal anti-LSD1 (Immunoblotting, ChIP-seq and ChIP-qPCR) BETHYL Cat# A300-215A rabbit polyclonal anti- OTUD7B (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) Proteintech Cat# 16605-1-AP mouse monoclonal anti- OTUD7B (immunoblotting) Proteintech Cat# 66276-1-Ig rabbit polyclonal anti-CoREST (Immunoblotting) CST Cat# 14567 mouse monoclonal anti- H3K9me2 (ChIP-seq and ChIP-qPCR) Abcam Cat#1220 rabbit polyclonal anti- H3K4me2 Millipore Cat# 07-030 rabbit polyclonal anti-HDAC1 (immunoblotting) Proteintech Cat# 10197-1-AP rabbit polyclonal anti-HDAC2 (immunoblotting) Proteintech Cat# 12922-3-AP rabbit polyclonal anti-USP22 (immunoblotting) Proteintech Cat# 55110-1-AP rabbit polyclonal anti-USP28 (immunoblotting) Proteintech Cat# 17707-1-AP rabbit polyclonal anti-Flag (immunoblotting) Proteintech Cat# 20543-1-AP rat monoclonal anti-HA (immunoblotting) Roche Cat# 3F10 mouse monoclonal anti-Actin (immunoblotting) Sigma Cat# A1978 mouse monoclonal anti-Myc (immunoblotting) CST Cat# 2276 rabbit polyclonal anti-Myc (immunoblotting) Proteintech Cat# 16286-1-AP mouse monoclonal anti-GFP (immunoblotting) Proteintech Cat# 66002-1-lg rabbit polyclonal anti-p62 (immunoblotting) Proteintech Cat# 18420-1-AP mouse monoclonal anti-Cyclin B (immunoblotting) CST Cat# 4135 mouse monoclonal anti-Cyclin E (immunoblotting) CST Cat# 4129 mouse monoclonal anti-Cyclin D1 (immunoblotting) Proteintech Cat# 60186-1-Ig rabbit polyclonal anti-CDK6 (immunoblotting) CST Cat# 14052-1-AP mouse monoclonal anti- Tubulin (immunoblotting) Proteintech Cat# 66240-1-lg rabbit polyclonal anti-PKD1 (immunoblotting) ABclonal Cat# A14200 rabbit polyclonal anti-RIN1 (immunoblotting) Proteintech Cat# 16388-1-AP rabbit polyclonal anti-RBBP8 (immunoblotting) Proteintech Cat# 12624-1-AP rabbit polyclonal anti-Keap1 (immunoblotting) Proteintech Cat# 10503-2-AP rabbit polyclonal anti-RASSF5 (immunoblotting) SAB Cat# 27727 rabbit monoclonal anti-Snail (immunoblotting) CST Cat# 3879 mouse monoclonal anti-His (immunoblotting) Proteintech Cat# 66005-1-Ig rabbit polyclonal anti-GST (immunoblotting) Proteintech Cat# 10000-0-AP mouse monoclonal anti-Lamin B1 (immunoblotting) Proteintech Cat# 66095-1-Ig mouse monoclonal anti- GAPDH (immunoblotting) Santa cruz Cat# sc32233 Table S6.
ChIP-qPCR:Article Title: OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis
Article Snippet: Antibodies list mouse monoclonal anti-LSD1 (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) CST Cat# 4218 rabbit polyclonal anti-LSD1 (Immunoblotting, ChIP-seq and ChIP-qPCR) BETHYL Cat# A300-215A rabbit polyclonal anti- OTUD7B (Immunoblotting, immunofluorescence, IHC and immunoprecipitation) Proteintech Cat# 16605-1-AP mouse monoclonal anti- OTUD7B (immunoblotting) Proteintech Cat# 66276-1-Ig rabbit polyclonal anti-CoREST (Immunoblotting) CST Cat# 14567 mouse monoclonal anti- H3K9me2 (ChIP-seq and ChIP-qPCR) Abcam Cat#1220 rabbit polyclonal anti- H3K4me2 Millipore Cat# 07-030 rabbit polyclonal anti-HDAC1 (immunoblotting) Proteintech Cat# 10197-1-AP rabbit polyclonal anti-HDAC2 (immunoblotting) Proteintech Cat# 12922-3-AP rabbit polyclonal anti-USP22 (immunoblotting) Proteintech Cat# 55110-1-AP rabbit polyclonal anti-USP28 (immunoblotting) Proteintech Cat# 17707-1-AP rabbit polyclonal anti-Flag (immunoblotting) Proteintech Cat# 20543-1-AP rat monoclonal anti-HA (immunoblotting) Roche Cat# 3F10 mouse monoclonal anti-Actin (immunoblotting) Sigma Cat# A1978 mouse monoclonal anti-Myc (immunoblotting) CST Cat# 2276 rabbit polyclonal anti-Myc (immunoblotting) Proteintech Cat# 16286-1-AP mouse monoclonal anti-GFP (immunoblotting) Proteintech Cat# 66002-1-lg rabbit polyclonal anti-p62 (immunoblotting) Proteintech Cat# 18420-1-AP mouse monoclonal anti-Cyclin B (immunoblotting) CST Cat# 4135 mouse monoclonal anti-Cyclin E (immunoblotting) CST Cat# 4129 mouse monoclonal anti-Cyclin D1 (immunoblotting) Proteintech Cat# 60186-1-Ig rabbit polyclonal anti-CDK6 (immunoblotting) CST Cat# 14052-1-AP mouse monoclonal anti- Tubulin (immunoblotting) Proteintech Cat# 66240-1-lg rabbit polyclonal anti-PKD1 (immunoblotting) ABclonal Cat# A14200 rabbit polyclonal anti-RIN1 (immunoblotting) Proteintech Cat# 16388-1-AP rabbit polyclonal anti-RBBP8 (immunoblotting) Proteintech Cat# 12624-1-AP rabbit polyclonal anti-Keap1 (immunoblotting) Proteintech Cat# 10503-2-AP rabbit polyclonal anti-RASSF5 (immunoblotting) SAB Cat# 27727 rabbit monoclonal anti-Snail (immunoblotting) CST Cat# 3879 mouse monoclonal anti-His (immunoblotting) Proteintech Cat# 66005-1-Ig rabbit polyclonal anti-GST (immunoblotting) Proteintech Cat# 10000-0-AP mouse monoclonal anti-Lamin B1 (immunoblotting) Proteintech Cat# 66095-1-Ig mouse monoclonal anti- GAPDH (immunoblotting) Santa cruz Cat# sc32233 Table S6.
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